Courses@CRG: Building minimal cells: from non-essential genes identification to genome engineering
The course will revolve around two axes: the identification of essential and non-essential genes in a given micro-organism; and the design, validation and implementation of genome engineering tools to produce genome-reduced cells. Participants will be trained in the production and sequencing of transposon libraries in bacteria, Tn-seq data analysis, and the in vitro rapid prototyping and testing of CRISPR-Cas9 genome edition tools. The course will feature a complete day of seminar, with didactic presentations by scientists at the forefront of their fields. This seminar will introduce the participants to the core concepts underlying the production of genome-reduced cells, in the model bacterium Bacillus subtilis and Mycoplasma genitalium. The next two days will be dedicated to hands-on tutorials and experiments in both wet and in silico biology.